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ColM resistance levels conferred by Cmi expression plasmids. BW25113 cells carrying either one of the cmi-expressing plasmids, pFFG11 (A) or pMLD232 (B), or the control vector, pET2160 (C), were grown as described in Materials and Methods. When the OD600 of the cultures reached 0.2, pure colicin M was added at various concentrations (the indicated values are in ng·ml−1).
