FIG. 6.
Determination of the transcription start sites of rpoH. Total RNAs from WT, ΔrpoE, ΔrseA, ΔmucD, and WT or ΔrpoE strains containing control plasmid p917 (−) or σE-overexpressing plasmid p917-rpoE (+) were used as templates in primer extension experiments. Total RNAs were obtained from cells incubated at 30°C or after a 60-min shift at 35°C. Primer rpoH-EXT was 5′ end labeled with 32P and extended with reverse transcriptase to map its corresponding gene promoter sequences. The arrows indicate the bands corresponding to the main start sites observed.