TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| B. subtilis strainsa | ||
| PY79 | Prototrophic wild type | 42 |
| RL1275 | ΔsigF::erm | Laboratory stock derived from MO173 (gift of P. Stragier) |
| AHB98 | ΔsigG::kan | 7 |
| AHB199 | ΔcsfB::tet | 7 |
| AHB1931 | Δfin::phleo | This study |
| AHB1983 | Δfin::phleo sacA::Pfin-fin kan | This study |
| AHB1933 | amyE::Pfin-lacZ cat | This study |
| AHB1956 | amyE::Pfin-lacZ cat ΔsigF::erm | This study |
| AHB1934 | amyE::Pfin-lacZ cat ΔsigG::kan | This study |
| AHB2085 | Δfin::phleo amyE::Pfin-gfp-fin spc | This study |
| AHB881 | amyE::PspoIIQ-lacZ cat | 6 |
| AHB1953 | amyE::PspoIIQ-lacZ cat Δfin::phleo | This study |
| AHB1985 | amyE::PspoIIQ-lacZ cat Δfin::phleo sacA::Pfin-fin kan | This study |
| AHB882 | amyE::PspoIIQ-lacZ cat ΔsigG::kan | 6 |
| AHB1954 | amyE::PspoIIQ-lacZ cat ΔsigG::kan Δfin::phleo | This study |
| AHB324 | ywrK::Tn917::amyE::PsspB-lacZ cat | 6 |
| AHB1952 | ywrK::Tn917::amyE::PsspB-lacZ cat Δfin::phleo | This study |
| AHB1984 | ywrK::Tn917::amyE::PsspB-lacZ cat Δfin::phleosacA::Pfin-fin kan | This study |
| AHB1879 | ywrK::Tn917::amyE::PsspB-lacZ cat ΔcsfB::tet | This study |
| AHB2112 | ywrK::Tn917::amyE::PsspB-lacZ cat Δfin::phleo ΔcsfB::tet | This study |
| Plasmids | ||
| pAH247 | phleo in pBluescript KS(+) | This study |
| pAH515 | amyE::Pfin-lacZ cat | This study |
| pAH537 | sacA::Pfin-fin kan | This study |
| pAH585 | amyE::Pfin-gfp-fin spc | This study |
a
All B. subtilis strains are isogenic with PY79.