Western blot analyses of protein fractions of P. gingivalis allele exchange mutants probed with anti-Rgp. Neg and Pos are the negative and positive controls, respectively. Fractions probed with anti-Rgp are indicated as follows: 698, RgpB(N698A); 695, RgpB(F695A); 693, RgpB(T693Q); OM, outer membrane; WC, whole cell.