TABLE 1.
Bacterial strains and plasmids
| Plasmid or strain | Descriptiona | Reference or source |
|---|---|---|
| Plasmids | ||
| pAL30 | ermF ligated into pGem-TEasy | 7 |
| pBH1.1 | pGem-TEasy::rgpB (nt 493-3166) | 20 |
| pUCtetQ | pUC18::tetQ | This study |
| pJY674F | pBH1.1-rgpB(Y674F) | This study |
| pJY674Y718F | pBH1.1-rgpB(Y674F Y718F) | This study |
| pJD696A | pBH1.1-rgpB(D696A) | This study |
| pKTG699P | pBH1.1-rgpB(G699P) | This study |
| pKTG716P | pBH1.1-rgpB(G716P) | This study |
| pKTK732Q | pBH1.1-rgpB(K732A) | This study |
| pKTT728QT730Q | pBH1.1-rgpB(T728Q T730Q) | This study |
| pKTTSTT | pBH1.1-rgpB(T675Q S679Q T682Q T684Q) | This study |
| pKTT724Q | pBH1.1-rgpB(T724Q) | This study |
| pKTERM1 | pUCtetQ::ermF | This study |
| pKTG6 | pKTERM1::rgpB(G699P) | This study |
| pKTG7 | pKTERM1::rgpB(G716P) | This study |
| pKTK1 | pKTERM1::rgpB(K732Q) | This study |
| pKTTT1 | pKTERM1::rgpB(T728Q T730Q) | This study |
| pKTB1 | pKTERM1::rgpB(nt 493-3166) | This study |
| pKTY1 | pKTERM1::rgpB(Y674F) | This study |
| pKTD1 | pKTERM1::rgpB(D696A) | This study |
| pKTYY | pKTERM1::rgpB(Y674F Y718F) | This study |
| pKTTSTT | pKTERM1::rgpB(T675Q S679Q T682Q T684Q) | This study |
| pKTT | pKTERM1::rgpB(T724Q) | This study |
| pCM1 | pGem-TEasy::rgpB(nt 493-2961)-SfoI | This study |
| pCM2 | pCM1::rgpB (nt 3015-3166) | This study |
| pCM3 | pKTERM::rgpBΔ(692-702) | This study |
| pCMT693Q | pKTERM1::rgpB(T693Q) | This study |
| pCMF695A | pKTERM1::rgpB(F695A) | This study |
| pCMN698A | pKTERM1::rgpB(N698A) | This study |
| pAL34 | 934-bp 5-prime fragment of pg0553 ligated between ApaI and AatII sites of pAL30 | This study |
| pAL34.1 | 1,122-bp 3-prime fragment of pg0553 ligated between SpeI and SalI sites of pAL34 | This study |
| P. gingivalis strains | ||
| W50AB | W50 rgpA::catrgpB::tetQ; Cmr Tcr | 40 |
| W50AB-Pos | W50 rgpA::catrgpB::tetQ::rgpB; Cmr Emr | This study |
| W50AB-Neg | W50 rgpA::catrgpB::tetQ::ermF; Cmr Emr | This study |
| W50AB-G699P | W50 rgpA::catrgpB::tetQ::ermF-rgpB(G699P); Cmr Emr | This study |
| W50AB-G716P | W50 rgpA::catrgpB::tetQ::ermF-rgpB(G716P); Cmr Emr | This study |
| W50AB-K732Q | W50 rgpA::catrgpB::tetQ::ermF-rgpB(K732P); Cmr Emr | This study |
| W50AB-T728T730Q | W50 rgpA::catrgpB::tetQ::ermF-rgpB(T728QT730Q); Cmr Emr | This study |
| W50AB-D696A | W50 rgpA::catrgpB::tetQ::ermF-rgpB(D696A); Cmr Emr | This study |
| W50AB-Y674F | W50 rgpA::catrgpB::tetQ::ermF-rgpB(Y674F); Cmr Emr | This study |
| W50AB-Y674Y718F | W50 rgpA::catrgpB::tetQ::ermF-rgpB(Y674F Y718F); Cmr Emr | This study |
| W50AB-T724Q | W50 rgpA::catrgpB::tetQ::ermF-rgpB(T724Q); Cmr Emr | This study |
| W50AB-TSTT | W50 rgpA::catrgpB::tetQ::ermF-rgpB(T675Q S679Q T682Q T684Q); Cmr Emr | This study |
| W50AB-T693Q | W50 rgpA::catrgpB::tetQ::ermF-rgpB(T693Q); Cmr Emr | This study |
| W50AB-F695A | W50 rgpA::catrgpB::tetQ::ermF-rgpB(F695A); Cmr Emr | This study |
| W50AB-N698A | W50 rgpA::catrgpB::tetQ::ermF-rgpB(N698A); Cmr Emr | This study |
| W50AB-Δ692-702 | W50 rgpA::catrgpB::tetQ::ermF-rgpBΔ(692-702); Cmr Emr | This study |
| ECR179 | E. coli JM109, pAL34 | This study |
| ECR179.1 | E. coli JM109, pAL34.1 | This study |
| ECR190 | P. gingivalis W50 pg0553::ermF | This study |
a
Tcr, tetracycline resistance; Cmr, chloramphenicol resistance; Emr, erythromycin resistance. Nucleotides (nt) are numbered according to GenBank accession number AF007124.