TABLE 3.
Mass spectrometric analysis data identifying PG0553
| Sample/peptidea | Experimentb | Mascot score | Expect value |
|---|---|---|---|
| W50AB-Neg | PMF | 73 | 1.1E−04 |
| W50AB-D696A | PMF | 81 | 1.9E−05 |
| 28QISFGGEPLSFSSR41 | MS/MS | 60 | 5.5E−05 |
| 56LTPDFNPEDLIAQSR70 | MS/MS | 85 | 1.2E−08 |
| 119AITLYYDAFNIPEGGR134 | MS/MS | 71 | 2.3E−07 |
| 329SDGLLLQLNDEVPLR343 | MS/MS | 52 | 1.8E−05 |
a
The identities of the four peptides were independently confirmed by MS/MS using the negative-control sample. All peptides matched to within 50 ppm of the calculated mass.
b
PMF experiments were conducted on the 63-kDa bands excised from the blot shown in Fig. 7A.