FIG. 5.
Effects of increased and decreased 5′ureB-sRNA expression on ureAB mRNA levels. The total RNAs from the H. pylori 43504 wild type, the transformant with pTM-PcagA-5′ureB-sRNA(+) that expressed a high level of 5′ureB-sRNA, the transformant with pTM-PcagA-5′ureB-sRNA(−) that expressed a low level of 5′ureB-sRNA, and control strains with pTM-PcagA were harvested after treatment at pH 7.4 for 30 min. RNA samples (5 μg) were separated in 6% polyacrylamide-urea gels and then transferred to a Zeta-Probe GT membrane. The ureAB transcripts were detected with a strand-specific oligonucleotide antisense probe (5′-ureB 2-1AS) that can also detect anti-5′ureB-sRNA, shown as a lower-molecular-mass band (top panels), and the 5′ureB-sRNA was detected with an oligonucleotide sense probe (5′-ureB 2-1S) complementary to the 5′ ureB (bottom panels). The gels (stained with ethidium bromide) are shown as loading controls (right panels).