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. 2010 Oct 29;193(1):296–300. doi: 10.1128/JB.01191-10

FIG. 1.

FIG. 1.

Effect of Pol IV (ΔdinB) and Pol II (ΔpolB and polBex1) on dnaX36 mutator activity. All strains are also mismatch repair deficient (mutL). Mutant frequencies were determined as described previously (1, 12). The strains used are lac-containing derivatives of MC4100 (5) into which the indicated dnaX, dinB, and polB alleles were introduced by P1 transduction as described previously (1, 5, 12). Each entry is based on at least 30 independent cultures. Average mutant frequencies with standard errors (SE) were determined using the statistical software program Prism (GraphPad). (A) Mutant frequencies for lac G·C → T·A transversions in the L (leading-strand) orientation. The mutant frequencies ± SE values were as follows: dnaX+, 1.3 ± 0.3; dnaX36, 11 ± 0.5; dnaX+ dinB, 1.2 ± 0.1; dnaX36 dinB, 9.7 ± 1; dnaX+ ΔpolB, 1.2 ± 0.2; dnaX36 ΔpolB, 18 ± 3; dnaX+ ΔpolB dinB, 1.4 ± 0.2; dnaX36 ΔpolB dinB, 12 ± 3; dnaX+ polBex1, 2.6 ± 0.3; dnaX36 polBex1, 235 ± 19; dnaX+ polBex1 dinB, 2.6 ± 0.4; dnaX36 polBex1 dinB, 250 ± 9. (B) Mutant frequencies for lac G·C → T·A transversions in the R (lagging-strand) orientation. The mutant frequencies ± SE values were as follows: dnaX+, 0.9 ± 0.2; dnaX36, 15 ± 1.2; dnaX+ dinB, 0.8 ± 0.2; dnaX36 dinB, 11 ± 0.7; dnaX+ ΔpolB, 0.8 ± 0.1; dnaX36 ΔpolB, 46 ± 6; dnaX+ ΔpolB dinB, 0.9 ± 0.2; dnaX36 ΔpolB dinB, 29 ± 5; dnaX+ polBex1, 8.9 ± 0.3; dnaX36 polBex1, 295 ± 30; dnaX+ polBex1 dinB, 5.1 ± 0.4; dnaX36 polBex1 dinB, 240 ± 13. (C) Mutant frequencies for lac A·T → T·A transversions in the L (leading-strand) orientation. The mutant frequencies ± SE values were as follows: dnaX+, 1.1 ± 0.3; dnaX36, 3.4 ± 0.6; dnaX+ dinB, 1.1 ± 0.2; dnaX36 dinB, 4.4 ± 0.5; dnaX+ ΔpolB, 1.0 ± 0.2; dnaX36 ΔpolB, 4.2 ± 0.3; dnaX+ polBex1, 2.2 ± 0.3; dnaX36 polBex1, 90 ± 7; dnaX+ polBex1 dinB, 2.0 ± 0.6; dnaX36 polBex1 dinB, 83 ± 5. ND, not done. (D) Mutant frequencies for lac A·T → T·A transversions in the R (lagging-strand) orientation. The mutant frequencies ± SE values were as follows: dnaX+, 0.8 ± 0.2; dnaX36, 4.1 ± 0.4; dnaX+ dinB, 0.8 ± 0.2; dnaX36 dinB, 3.1 ± 0.4; dnaX+ ΔpolB, 0.7 ± 0.2; dnaX36 ΔpolB, 5.1 ± 0.4; dnaX+ polBex1, 3.3 ± 0.3; dnaX36 polBex1, 200 ± 11; dnaX+ polBex1 dinB, 3.0 ± 0.4; dnaX36 polBex1 dinB, 133 ± 9. ND, not done.