FIG. 6.

Differentiation and antibiotic-producing phenotypes of ΔsoxR. (A) Differentiation progress of wild-type (M145 with pSET162), ΔsoxR with pSET162, ΔsoxR complemented with pSET162::soxR, and ΔactII-ORF4 (M511) strains on R2YE solid plates. Formation of aerial mycelia, spores, and pigmented antibiotics was examined visually by taking photographs at 2, 4, and 6 days after inoculation. (B) Quantification of actinorhodin (Act) production. Cells from the M145 and ΔsoxR strains with the parental pSET162 plasmid, the ΔsoxR mutant complemented with pSET162::soxR, and M511 were grown in R2YE liquid medium for various lengths of time from 19 to 138 h. Average values and standard deviations from triplicate cultures are presented. (C) Quantification of undecylprodigiosin (Red) in cells prepared as for panel B.