FIG. 2.

SgrR-dependent activation of setA expression. (A) Genetic organization of the sgrR-sgrS region. The directions of transcription of the sgrR and sgrS genes are depicted by the arrows at the top. The −35 and −10 promoter elements are indicated by horizontal lines above the nucleotide sequence. The boxed nucleotides show the positions where mutations sub1 and sub2 were constructed. The substituted sequences are shown below the wild-type sequence. (B) Cells were grown in LB, and 0.1% αMG was added to half of the cultures. β-Galactosidase activity was assayed 45 min after the addition of αMG. The error bars indicate the standard deviations of three independent experiments.