FIG. 7.

Effects of setA mutation on the efflux of [¹⁴C]αMG. Wild-type (DJ480), ΔsetA::cm mutant (ST101), and ΔmanXYZ::kan ΔptsG::cm mutant (CV107) strains were grown at 37°C in minimal MOPS medium supplemented with 0.4% glycerol to an OD₆₀₀ of ∼0.1. The cells were incubated with [¹⁴C]αMG (3.3 μM; 1 μCi/ml) at room temperature for 20 min and then diluted 200-fold with fresh minimal MOPS-glycerol medium. Radioactivity was examined at the indicated times as described in Materials and Methods. The error bars indicate the standard deviations of three independent experiments.