TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| MG1655 | Wild-type E.coli K-12 | D. Jin (NCI) |
| DJ480 | MG1655 Δlac X74 | D. Jin (NCI) |
| BAH100 | DJ480 imm21sgrS′-lacZ | This study |
| BAH101 | DJ480 imm21sgrS′-lacZ, ΔsgrR::cm | This study |
| CS168 | DJ480 λattB::lacIqtetR Spr | This study |
| CV107 | DJ480 ΔptsG ΔmanXYZ ΔsgrS | C. K. Vanderpool (NIH) |
| CV700 | DJ480 ΔsgrR::cm | 33 |
| ST101 | DJ480 ΔsetA::cm | This study |
| YS131 | DJ480 setA′-lacZ | This study |
| YS133 | DJ480 setA′-lacZ ΔsgrR::cm | This study |
| YS135 | DJ480 sub1 | This study |
| YS137 | DJ480 sub2 | This study |
| YS140 | DJ480 setA′-lacZ sub1 | This study |
| YS142 | DJ480 setA′-lacZ sub2 | This study |
| YS143 | DJ480 λattB::lacIqtetR Spr ΔsetA::cm | This study |
| YS144 | DJ480 λattB::lacIqtetR SprsgrS1 | This study |
| YS214 | DJ480 imm21sgrS′-lacZ ΔsetA::cm | This study |
| YS215 | DJ480 imm21sgrS′-lacZ ΔsgrS | This study |
| YS219 | DJ480 setA′-lacZ ΔkdgR::FRT | This study |
| YS224 | DJ480 imm21sgrS′-lacZ sgrS1 | This study |
| YS225 | DJ480 imm21sgrS′-lacZ ΔkdgR::FRT | This study |
| YS228 | DJ480 imm21sgrS′-lacZ Δcrp::cm | This study |
| YS231 | DJ480 setA′-lacZ Δcrp::cm | This study |
| Plasmids | ||
| pZE21 | Vector control for plasmid pZEYS1 | 18 |
| pZEYS1 | pZE21 with setA under the control of the PLtetO-1 promoter | This study |
| pHDB3 | Vector control for plasmid pBRBAH3 | 31 |
| pBRBAH3 | pHDB3 with sgrR1 | This study |