TABLE 1.
Bacillus subtilis strains and plasmids used in this study
| Strain or plasmid | Relevant genotyped | Reference or transformation in this study |
|---|---|---|
| YB955 | hisC952 metB5 leuC427 xin-1 SpβSENS | 49 |
| HB2078 | perR::kan Kanr | 15 |
| YB9102 | ΔsigB::Cmr | 49 |
| PERM598 | 168 ΔmutY::SpSpr | 55 |
| PERM668c | hisC952 metB5 leuC427 xin-1 SpβSENS pMUTIN4::mutY Emr | pPERM666→YB955b |
| PERM704c | ΔmutY::SpSpr | PERM598→YB955a |
| PERM739c | ΔmutSL::NeoNeor | 36 |
| PERM828c | ΔmutSL::NeoΔmutY::SpNeor Spr | PERM739→PERM704a |
| PERM896 | ΔmutSL::Neo pMUTIN4::mutY Emr Neor | pPERM666→PERM739b |
| PERM897 | perR::kan pMUTIN4::mutY Emr Kanr | pPERM666→HB2078b |
| PERM898c | ΔsigB::Cm pMUTIN4::mutY Emr Cmr | pPERM666→YB102b |
| PERM899 | ΔmutY::Sp mutant with a Phs-mutY construct inserted into the amyE locus; Spr | pPERM852→PERM704b |
| PERM795c | hisC952 metB5 leuC427 xin-1 SpβSENS pMUTINFLAG::mutY Emr | pPERM790→YB955b |
| PERM947 | ΔmutSL::Neo pMUTINFLAG::mutY Emr Neor | pPERM790→PERM739b |
| pPERM626 | pCR-BluntII-TOPO with a 319-bp BamHI/HindIII PCR product containing a mutY fragment; Kanr | This study |
| pdr-111-amyE-Phyperspank | bla-and spec-carrying Phyperspank promoter | 37 |
| pPERM707 | pCR-BluntII-TOPO with a 1,107-bp HindIII/KpnI PCR product containing mutY; Kanr | This study |
| pPERM735 | pCR-BluntII-TOPO with a 1,157-bp HindIII/SalI PCR product containing mutY; Kanr | This study |
| pPERM790 | pMUTIN-FLAG carrying a 1,107-bp HindIII/KpnI fragment from pPERM707; Emr | This study |
| pPERM852 | pdr-111-amyE-Phyperspank carrying a 1,157-bp HindIII/SalI fragment from pPERM735; Spr | This study |
| pPERM666 | pMUTIN4 carrying a 319-bp BamHI/HindIII DNA fragment encompassing 208 bp upstream and 111 bp downstream of the mutY translational start codon; Emr | This study |
a
Chromosomal DNA from the strain to the left of the arrow was used to transform the strain to the right of the arrow.
b
Plasmid DNA from the strain to the left of the arrow was used to transform the strain to the right of the arrow.
c
The background for this strain is YB955.
d
Cm, chloramphenicol; Em, erythromycin; Kan, kanamycin; Neo, neomycin; Sp, spectinomycin.