TABLE 2.
Spontaneous mutation frequencies of mutSL- and mutY-deficient strainsa
Strain | Mean Rifr mutation frequency ± SD (10−9)b | Relative frequency |
---|---|---|
YB955 | 1.6 ± 0.92 | 1 |
mutYmutant | 13.74 ± 0.217 | 8.58 |
mutY::amyE-Phs-mutY mutant | 129.145 ± 18.52 | 80.71 |
mutSL mutant | 106.67 ± 2.003 | 66.66 |
mutY mutSL mutant | 74.53 ± 1.94 | 46.58 |
Cells were grown for 12 h at 37°C in antibiotic A3 medium (Difco) supplemented with the proper antibiotics, and aliquots were plated on six LB plates containing 10 μg/ml rifampin. Rifr colonies were counted after 1 day of incubation at 37°C.
Values shown are averages for three independent experiments ± standard deviations.