FIG. 4.

Type II hCGβ promoter analysis. (A) Schematic of hCGβ1/2 promoter, 5′ UTR, and first exon. The type II insert is demarcated by the dotted line. The extent of hCGβ1/2 sequence included in reporter constructs β1/2L (white bar), β1/2LΔ (hatched bar), β1/2S (black bar), and β1/2SΔ (gray bar) is shown below. (B) Relative luciferase activity in HeLa S3 cells transfected with the β1/2L and β1/2S constructs and their corresponding snaR-G deletion constructs diagrammed in panel A. Activity is normalized to β1L construct, and standard deviations (n = 4 duplicates) are shown. Two-tailed Student's t test: *, P < 0.002, and **, P < 0.001. (C) Northern blots of total RNA from transfected JAr cells. (Top) Cells were transfected with β1L (0, 0.5, 1, or 2 μg) or β1LΔ (2 μg) as indicated, and blots were probed for snaR-G1. (Bottom) As in top panel except that cells were transfected with β2L or β2LΔ and blots were probed for snaR-G2. (D) As in panel C, except that HeLa S3 cells were transfected with 0, 0.3, 0.75, 1.5, or 3 μg of β1L or β2L or with 3 μg of β1LΔ or β2LΔ.