TABLE 1.
Type II mRNA start codon usage in vivo
| Construct | AUG order | Reading frame | Observed banda | Molecular mass (kDa) |
AUG context | |
|---|---|---|---|---|---|---|
| Predicted | Observedb | |||||
| FFL | ++++ | 60.7 | 60.5 | ACCaugG | ||
| β1a/2a | 1 | 1 | − | 69.8 | TCCaugT | |
| 2 | 2 | +++ | 68.0 | 68.6 | GACaugT | |
| 3 | 2 | ++ | 66.6 | 66.1 | AGCaugG | |
| 4 | 1 | + | 66.3 | 66.4 | GACaugG | |
| 5 | 3 | − | TCAaugC | |||
| β2b′ | 1 | 1 | − | 68.4 | TCCaugT | |
| 3 | 1 | + | 65.0 | 65.2 | AAGaugG | |
a
Expression levels based on anti-FFL antibody immunoblot analysis as in Fig. 5D. −, no detectable expression; + to ++++, low to high expression.
b
Apparent molecular masses were calculated using GeneTools software (v4.01; SynGene) calibrating against unmodified FFL protein and molecular mass markers (Precision Plus; Bio-Rad).