Skip to main content
. 2010 Nov 1;31(2):365–374. doi: 10.1128/MCB.00918-10

FIG. 3.

FIG. 3.

Internal box C/D RNP-guided nucleotide methylation requires the terminal box C/D RNP. (A) Schematic presentation of the chimeric hU24 (box C/D)-snR5 (box H/ACA) snoRNA. The bipartite structure of snR5 with conserved H and ACA boxes (enclosed) is shown. Box C/D snoRNA inserts are positioned between two NcoI restriction sites indicated with arrows. (B) Schematic presentations of the box C/D portion of the chimeric hU24-snR5 snoRNAs are shown above the respective primer extension gel lanes. WT and blank lanes correspond to yeast cells transformed with no plasmid and plasmid lacking the hU24-snR5 chimeric insert, respectively. Primer extension stop sites for the box C/D (D) and C′/D′ (D′) RNP-guided nucleotide methylations are indicated on the left. Altered methylation levels with respect to wild-type U24 are indicated below the respective gel lanes.