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Substrate supercoiling influences the kinetics of excision. (A) The kinetics of transposon excision were analyzed using the inverted-repeat substrate (pRC1181) with superhelical densities ranging from −0.06 to +0.02. A photograph of an ethidium bromide-stained agarose gel is shown. (B) The backbone product in panel A was quantified using a Fluorimager, normalized to the maximum level achieved with the substrate with a σ of −0.06, and plotted.
