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. 2010 Nov 15;31(2):256–266. doi: 10.1128/MCB.00717-10

FIG. 4.

FIG. 4.

Phosphorylation of TTP by MK2 inhibits TTP-mediated mRNA deadenylation in cells. (A) Northern blot assays showing decay rates of β-6bs in the presence of tethered wild-type TTP (WT) or TTP-AA, in which serines 52 and 178 have been mutated to alanines. Cells were transfected with TTP expression vectors in the presence of no exogenous kinase (−), a constitutively active mutant kinase, MK2EE, or a kinase-dead mutant kinase, MK2KR, as indicated on the left. Calculated β-6bs mRNA t1/2s and average n-fold changes (average of three experiments) in the presence of phosphorylated TTP relative to nonphosphorylated TTP are given on the right. (B) Northern blot assays of mRNA decay assays as in panel A but without (lanes 1 to 7) or with (lanes 8 to 12) coexpression of the catalytically inactive form of the human decapping enzyme hDcp2-E148Q in the presence of MS2-TTP and MK2EE (top panel) or MK2KR (bottom panel).