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. 2010 Nov 10;85(2):1011–1024. doi: 10.1128/JVI.01092-10

FIG. 6.

FIG. 6.

Infectivity assays with Bo10 Del BoHV-4 mutant. (A) MDBK cells were exposed to eGFP-expressing (eGFP +) wild-type virus (WT eGFP) or Bo10 Del BoHV-4 strain (0.5 PFU/cell) for the times indicated and then washed with PBS and cultured overnight. Viral infection was assayed 18 h later by flow cytometry for eGFP expression. The data presented are the average ± SEMs for triplicate measurements. The data were analyzed by two-way ANOVA and Bonferroni posttests. The values for WT eGFP-infected cells were significantly different (P < 0.001) from the values for the Bo10 Del strain as indicated (***). (B) MDBK cells were exposed to WT eGFP or Bo10 Del BoHV-4 strain (0.5 PFU/cell) for the times indicated and then washed either with PBS (pH 7.4) or with isotonic (pH 3) buffer (acid wash). Viral infection was assayed by measuring eGFP expression 18 h later as for panel A.