FIG. 7.

Expression of pUL21a in trans complements the growth of UL21a deletion virus. MRC-5 cells were transduced with retrovirus expressing GFP (MRC5-GFP) or expressing the GFP/UL21a fusion protein (MRC5-GFP/UL21a) and then infected with ADwt and ADsubUL21a at an MOI of 0.2. Cell-free viruses were collected at different days postinfection, and their yields were determined by TCID₅₀ assay in HFFs (A). In addition, the titers of ADwt and ADsubUL21a collected at 12 dpi from MRC5-GFP/UL21a cells were also determined on both MRC5-GFP and MRC5-GFP/UL21a cells to compare their titers measured on complementing and noncomplementing cells (B). Error bars indicate standard deviations.