TABLE 1.

Primers and probes used for RT-qPCR

Transcript	qPCR type	Primers	6FAM-TAMRA TaqMan probe
UL122-123 pre-mRNA	SYBR green	5′-TCTGCCAGGACATCTTTCTCG-3′, 5′-GGAGACCCGCTGTTTCCAG-3′	Not applicable
UL123 (IE1)	TaqMan	5′-CAAGTGACCGAGGATTGCAA-3′, 5′-CACCATGTCCACTCGAACCTT-3′	5′-TCCTGGCAGAACTCGTCAAACAGA-3′
UL122 (IE2)	SYBR green	5′-TGACCGAGGATTGCAACGA-3′, 5′-CGGCATGATTGACAGCCTG-3′	Not applicable
UL37	TaqMan	5′-CCAAGGCGGGAGAGGAT-3′, 5′-CTGGCCTTTTGGTACTTTAGCT-3′	5′-TTCAAGGCGTTTTCGCTGGATCC-3′
TRS1	SYBR green	5′-CTGTGCAAATGTGGAAGATACCT-3′, 5′-GTCCAGTCCCAGAGCTTGAG-3′	Not applicable