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. 2010 Nov 17;85(2):705–714. doi: 10.1128/JVI.01557-10

FIG. 5.

FIG. 5.

Impact of HCMV infection on CaMKK1 mRNA and protein levels. (A) Analysis of CaMKK1 mRNA during HCMV infection by qPCR. Serum-starved MRC-5 human fibroblasts were mock infected or infected with HCMV (MOI = 3). Cellular mRNA isolated from cells harvested at 24, 48, and 72 h postinfection was processed for qPCR using CaMKK1-specific primers. Values are plotted as mean relative abundances ± SE observed after normalization to GAPDH (n = 3). (B) Analysis of CaMKK1 protein accumulation over a time course of HCMV infection. Serum-starved MRC-5 human fibroblasts were mock infected or infected with HCMV (MOI = 3). Cells were harvested at 4, 24, 48, and 72 h postinfection and analyzed by Western blotting with two independent CaMKK1-specific antibodies. Tubulin was also blotted for as a loading control.