TABLE 1.
Surface expression of tetherin and its derivatives used in this studya
| Name of N-terminally KGC-tagged fusion protein | MFI of tetherin |
|---|---|
| Untagged hu-tetherin | 107.6 ± 12.7 |
| hu-tetherin | 117.6 ± 16.2 |
| KGCstop | 13.1 ± 6.8 |
| mo-tetherin | ND |
| mo(huTM) | ND |
| mo(huECL) | ND |
| mo(huCC) | 123.3 ± 6.3 |
| mo(huGPI) | ND |
| hu(muTM) | 115.3 ± 4.7 |
| hu(muECL) | 138.4 ± 15.0 |
| hu(muCC) | ND |
| hu(muGPI) | 127.9 ± 9.8 |
| LLL(22-24)AAA | 116.3 ± 14.6 |
| GIG(25-27)AAA | 138.6 ± 27.2 |
| ILV(28-30)AAA | 159.4 ± 17.7 |
| LLI(31-33AAA | 160.2 ± 19.0 |
| IVI(34-36)AAA | 142.1 ± 17.9 |
| LGV(37-39)AAA | 161.0 ± 14.1 |
| PLI(40-42)AAA | 113.9 ± 12.3 |
| IFT(43-45)AAA | 149.8 ± 8.5 |
| IKA(46-48)AAA | 117.9 ± 2.0 |
| I34A | 155.9 ± 5.9 |
| V35A | 135.1 ± 34.5 |
| I36A | 157.1 ± 18.8 |
| L37A | 141.6 ± 26.8 |
| G38A | 169.1 ± 23.6 |
| V39A | 164.5 ± 14.5 |
| P40A | 165.9 ± 4.1 |
| L41A | 140.1 ± 9.8 |
| I42A | 143.6 ± 29.1 |
a
Values indicate the means from three independent experiments plus standard deviations. ND, not detected