Skip to main content
. 2010 Nov 10;85(2):742–752. doi: 10.1128/JVI.02087-10

FIG. 2.

FIG. 2.

Modulation of cellular receptors by primate lentivirus nef alleles. PBMCs were transduced with HIV-1 NL4-3 Nef-eGFP constructs coexpressing the indicated nef alleles and eGFP and assayed for surface expression of CD4, CD3, CD28, MHC-I, and CXCR4. The HIV-1 NL4-3, NA7, and SIVmac239 nef alleles have been functionally characterized in previous studies (3, 50) and are shown for comparison. Nef-mediated modulation of the indicated cellular receptors was calculated by dividing the mean fluorescence intensity obtained on cells coexpressing eGFP and the different nef alleles by the mean fluorescence obtained for cells transduced with a control HIV-1 construct containing a disrupted nef gene. The same ranges of eGFP expression were used in all calculations. Values give averages ± SD derived from three independent experiments.