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. 2010 Nov 10;85(2):742–752. doi: 10.1128/JVI.02087-10

FIG. 3.

FIG. 3.

Interaction of Nef with the human and rhesus macaque TCR-CD3-ζ chains. (A) Alignment of the human CD3 sequence with those of other primate species. Dots represent amino acid identity; dashes represent gaps. The two domains previously proposed to interact with Nef (49) are indicated. (B) Expression levels of CD8 fusions containing CD3-ζ cytoplasmic domains of human (hu) or rhesus macaque (rh) origin. 293T cells were transiently cotransfected with pCGCG vectors expressing eGFP alone (control) or together with CD8-CD3-ζ fusion proteins and proviral HIV-1 constructs containing the nef alleles or a disrupted nef gene (nef*). The cells were stained for CD8 at 2 days posttransfection. (C) Levels of CD8 cell surface expression in the presence of the indicated nef alleles compared to those measured in the absence of Nef (100%). Values give averages ± SD derived from two independent experiments. Similar results were obtained with 5 different SIVolc nef alleles.