FIG. 7.

Enhancement of viral replication by primate lentivirus nef alleles. (A) Replication kinetics of recombinant NL4-3 variants containing the indicated nef alleles in PBMCs. Infections were carried out using virus stocks containing 1 ng p24 antigen. The numbers of virally infected GFP⁺ cells were measured by flow cytometric analysis. Similar results were obtained in two independent experiments. (B) Cumulative numbers of HIV-1-infected GFP⁺ cells detected in PBMC cultures at days 3, 5, 7, and 10 postinfection. Shown are averages ± SD derived from three independent infections.