FIG. 7.

Sequence alignment of several T-Ag OBDs. The programs Clustal W (27) and Jalview (50) were used to calculate and display the sequence alignment of several representative T-Ag OBDs. The amino acids are colored by sequence identity (a color gradient from dark blue, indicating 100% identity, to white, indicating 0% identity). The red boxes indicate the location of the four residues identified as being important in ssDNA binding in this study; the SV40 T-Ag amino acid numbers are also presented. The sequences used in this alignment are from the polyomavirus members SV40, BK, JC, mouse (Mus), and Merkel cell carcinoma.