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. 2010 Nov 10;85(2):725–732. doi: 10.1128/JVI.01226-10

FIG. 2.

FIG. 2.

Analysis of interactions between the N- and C-terminally truncated L and P, V, NP, or L proteins by immunoprecipitation and Western blot assay. (A) Schematic diagram of the N- and C-terminally truncated L proteins. Binding to P, V, NP, or L protein is summarized at right. (B to E) The proteins from transfected BSR T7/5 cell lysates were analyzed by Western blotting for anti-P/V MAb (B and C), anti-NP MAb (D), or anti-L MAb (E) (top panels) and for anti-Flag polyclonal antibody (middle panels). Immunoprecipitates with anti-P/V MAb (B and C), anti-NP MAb (D), or anti-Flag polyclonal antibody (E) were probed by anti-Flag polyclonal antibody or anti-L MAb in Western blot assays (bottom panels). Numbers on the bottom correspond to the L proteins depicted in panel A. Sizes of molecular mass markers (in kDa) are shown.