Infection with pseudotyped viruses utilizing patient-derived env. CD4+ T cells (n = 100,000) isolated from a randomly selected subset of HIV-1-negative donors (triangles), ES (diamonds), and viremic patients (circles) were infected with 32 ng p24 equivalents of virus pseudotyped with three independent plasma and proviral envelope genes from two patients with progressive HIV-1 disease. Each symbol represents cells from an individual patient which were infected with one of three different viruses pseudotyped with env from three different primary HIV-1 isolates (black, white, or gray).