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. 2010 Nov 10;85(2):873–882. doi: 10.1128/JVI.02062-10

FIG. 5.

FIG. 5.

Effect of TTSP expression on ACE2 and HIV-SARS S transductions. (A) Cells (106) transfected with a constant 1 μg of ACE2 and increasing doses of the indicated TTSPsor pCAGGS empty vector (EV) were analyzed at 2 days posttransfection by IB for the C9 epitope appended to the ACE2 C terminus. (B) Cells transfected with 1 μg of ACE2C9 and the indicated amounts of TMPRSS2 were analyzed at 2 days posttransfection with anti-C9 and anti-FLAG tag antibodies, respectively. (C) Parallel unlysed cell cultures were transduced with HIV-SARS S pseudoviruses, and luciferase accumulations were measured at 40 h posttransduction. FL, firefly luciferase. The values to the right of panels A and B are molecular sizes in kilodaltons.