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. 2010 Nov 10;85(2):873–882. doi: 10.1128/JVI.02062-10

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FIG. 7. — Interaction between TMPRSS2 and ACE2. (A) 293T cells transfected with pCAGGS-TMPRSS2_FLAG (0.5 μg per 10⁶ cells) and pcDNA3.1-ACE2_C9 (1 μg per 10⁶ cells), individually or in combination, were lysed and subsequently incubated with rabbit anti-FLAG, mouse anti-C9, or mouse IgG antibody on protein G magnetic beads. Eluted proteins were analyzed by IB using the indicated antibodies. (B) Lysates from 293T cells transfected with pCAGGS-TMPRSS2_FLAG (0.5 μg per 10⁶ cells) or pCAGGS-TMPRSS2(S441A)_FLAG (0.5 μg per 10⁶ cells), individually or in combination with pcDNA3.1-ACE2_C9 (1 μg per 10⁶ cells), were subjected to IPs using rabbit anti-FLAG antibody. Eluted proteins were analyzed by IB using mouse anti-C9 antibody. The values to the left are molecular sizes in kilodaltons.