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. 2010 Nov 10;85(2):957–967. doi: 10.1128/JVI.01688-10

FIG. 8.

FIG. 8.

Leading (LE) and lagging (LA) strand synthesis with wild-type and exo− polymerase. (A) All assays contained 10 nM MC70/TP, 8 mM Mg(OAc)2, 100 nM UL30-UL42, 200 nM UL5-UL8-UL52, 40 μM dATP and dTTP, 100 μM dGTP, 100 μM dCTP, 2.8 mM ATP, and 0.8 mM GTP, CTP, and UTP. The ICP8 concentration was 330 nM. (B) The reaction mixtures contained 10 mM MgCl2 instead of the Mg(OAc)2 to determine if the longer lagging strand products are solely due to the higher dNTP concentrations or are also due to the altered salt conditions.