TABLE 1.
Amount of DNA produced in 45 min in assays containing 10 nM of the designated DNA template and MgOAc or MgCl2a
| DNA template | Treatment | MC70/TP replication |
MC140/TP replication |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Leading strand (pmol/μl) |
Lagging strand (pmol/μl) |
Leading strand (pmol/μl) |
Lagging strand (pmol/μl) |
||||||
| No ICP8 | With ICP8 | No ICP8 | With ICP8 | No ICP8 | With ICP8 | No ICP8 | With ICP8 | ||
| HSV exo+ | MgOAc | 5.2 | 8.4 | 1.7 | 2.5 | 4.7 | 12 | 3.1 | 2.7 |
| HSV exo− | MgOAc | 8.8 | 16 | 4.8 | 11 | 9.1 | 17 | 4.1 | 7.2 |
| HSV exo− | MgCl2 | 6.2 | - | 6.7 | - | ||||
a
Mixtures also contained the following: 40 μM dATP and dTTP; 100 μM dGTP; 100 μM dCTP; 3 mM ATP; 1 mM GTP, CTP, and UTP; 8 mM MgOAc or 10 mM MgCl2; 100 nM UL30-UL42; 200 nM UL52-UL5-UL8; and 330 nM ICP8.