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. 2010 Nov 10;85(2):865–872. doi: 10.1128/JVI.01412-10

FIG. 1.

FIG. 1.

Size exclusion chromatography of recombinant HA63-286-RBD. The isolated HA receptor-binding domain elutes as a well-folded monomer (labeled black peak) with an apparent molecular size of ∼25 kDa compared to molecular size standards (labeled gray peaks) on a Superdex 200 size exclusion chromatography column. The purified peak was collected and yielded well-diffracting crystals. Abs, absorbance.