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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Biochimie. 2010 Oct 26;93(2):361–368. doi: 10.1016/j.biochi.2010.10.009

Figure 2. Hypoxia induces activation of pRL-SV40, pRL-CMV, and pRL-TK constitutive reporters.

Figure 2

Rcho-1 trophoblast cells were plated at equal cell number of 1.0 × 105 cells and transfected with 1 μg of PGK-1-HRE and 0.2μg of pRL-SV40, or pRL-CMV or pRL-TK as indicated using the conventional transfection method. Samples were incubated at 5% or 21% oxygen for 18hrs and analyzed for activation of the constitutive reporter. Results are the average of three independent experiments. Luciferase activity at each concentration was analyzed and is shown relative to activity at 21% oxygen. Error bars represent standard deviation. Significance was denoted as *p≤0.01 or **p≤0.001 and was determined using a one way Anova with Tukeys post hoc test.