Fig. 8. Sid1p SPB localization coincides with Cdk inactivation and cyclin destruction. (A) nda3-km311 GFP–sid1 cells were blocked in early mitosis by incubation at 19°C for 6 h, released from the block by shifting to 30°C and samples were collected every 10 min and scored for percentage anaphase, septation, GFP–Sid1p localization and H1 kinase activity. (B) GFP–sid1 cells were fixed and stained for tubulin and cyclin (Cdc13p). Numbers indicate the cell cycle stages described in the text. (C) cdc7-HA cells were fixed and stained for Cdc7-HA and cyclin.