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. 2010 Nov 26;30(1):154–164. doi: 10.1038/emboj.2010.288

Figure 5.

Figure 5

SAXS envelopes and models of SlmA-FtsZ complexes. (A) The average SlmA–FtsZ envelope, as determined by DAMAVER, is displayed as grey spheres (Svergun, 1999; Svergun et al, 2001). The model of the SlmA–FtsZ complex was calculated by BUNCH (Petoukhov and Svergun, 2005). In this model, a SlmA dimer (yellow) is flanked by two FtsZ molecules (turquoise and magenta). (B) The SAXS envelope of the SlmA–(FtsZ–GFP) complex was calculated as in A. Compared with the SlmA–FtsZ envelope (A) additional density was clearly observed, which corresponded to the GFP (green) fused at the C terminus of FtsZ(1–316). (C) SAXS structure of SlmA–FtsZ showing that when bound to SlmA, FtsZ protofilaments can form, but emanate in opposite directions relative to each other. The two FtsZ oligomers (cyan and magenta) in the structure flank the SlmA dimer (yellow).