Figure 2.

Individual and synergistic activities of eIFs 4A/4B/4G, DHX29, Ded1 and DDX3 in promoting scanning through 5′-UTRs with an internal stem of moderate stability. (A–E) Toe-printing analysis of 48S complex formation on (A–D) Stem-1 and (E) low-energy Stem mRNAs in the presence of indicated combinations of factors. Initiation codons and the positions of full-length cDNAs and of assembled ribosomal complexes are shown on the sides of each panel. Lanes C/T/A/G depict corresponding DNA sequences.