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. 2010 Nov 26;30(1):115–129. doi: 10.1038/emboj.2010.302

Figure 4.

Figure 4

48S complex formation on mRNAs with AUG triplets downstream of stable stems depending on the presence of eIF1 and DHX29. Toe-printing analysis of 48S complex formation on (A–C) mRNAs-containing AUG codons 10, 21 and 30 nucleotides downstream of Stem-2, respectively, (D) mRNA-containing AUG triplets 15 nts downstream of the 5′-end of mRNA, in the loop of Stem-2, and 10 nts downstream of the stem, and (E) mRNA containing an additional GC-rich stem at the extreme 5′-end and two AUG triplets, in the loop of Stem-2 and 10 nts downstream from the stem, respectively, in the presence of indicated combinations of factors. Initiation codons and the positions of full-length cDNAs and of assembled ribosomal complexes are shown on the sides of each panel. Lanes C/T/A/G depict corresponding DNA sequences.