| p630 |
Wild type GCN2, 2µ, URA3
|
Wek et al. (1990) |
| p2326 |
gcn2-K628R in p630 backbone |
pB34 in Romano et al. (1997)
|
| pB82 |
gcn2-Δ572–999 in p630 backbone |
Qiu et al. (1998) |
| p733 |
gcn2-Δ1161–1245 in p630 backbone |
Wek et al. (1989) |
| p732 |
gcn2-Δ1401–1535 in p630 backbone |
Wek et al. (1989) |
| pB106 |
gcn2-Δ1246–1400 in p630 backbone |
this study |
| pB47 |
gcn2-Δ1536–1659 in p630 backbone |
Qiu et al. (1998) |
| p722 |
GCN2, CEN6, ARSH4, URA3
|
Wek et al. (1989) |
| pB54 |
GCN2-myc, unique SacI (5′) and MluI (3′) sites flanking a myc tag inserted betweencodons 1 and 2 of GCN2 in p722 |
this study |
| pB121 |
GCN2-myc from pB54 in p630 backbone |
this study |
| pB123 |
gcn2-Δ10–109, unique SacI between codon 1 and 2 of GCN2 and deletion marked withMluI at the deletion junction in pB121 backbone; the myc tag is not present in this construct |
this study |
| pB101 |
gcn2-Δ237–322, unique SacI between codon 1and 2 of GCN2 and deletion marked withMluI at the deletion junction in pB121 backbone; the myc tag is not present in this construct |
this study |
| pB29 |
gcn2-Δ110–235 in p630 backbone |
this study |
| pB32 |
gcn2-Δ324–538 in p630 backbone, with a unique SacI site at the deletion junction |
this study |
| pB149 |
gcn2-Δ324–538 in p630 backbone, with unique SacI (5′) and MluI (3′) sites at thedeletion junction, separated by two codons encoding Leu and Ala |
this study |
| pB152 |
gcn2-Δ184–237, codons 184–237 flanked by unique SacI (5′) and MluI (3′) sites introducedinto pB149 |
this study |
| pB130 |
GST–GCN2(1–598) in pGEX-5X-1 (Pharmacia Biotech.) |
this study |
| pB131 |
GST–GCN2(1–272) in pGEX-5X-1 |
this study |
| pB132 |
GST–GCN2(1–110) in pGEX-5X-1 |
this study |
| pB133 |
GST–GCN2(108–272) in pGEX-5X-1 |
this study |
| pHQ551 |
GST–GCN2(568–998) in pGEX-5X-1 |
Qiu et al. (1998) |
| pHQ531 |
GST–GCN2(1498–1659) in pGEX-5X-1 |
Qiu et al. (1998) |
| pHQ530 |
GST–GCN2(970–1497) in pGEX-5X-1 |
gift from Dr H.Qiu |
| pB165 |
GST–GCN2(1–598), myc tagged at the 3′ end and under the GAL1 promoter in pEGKT |
this study and Mitchell et al. (1993)
|
| pB202 |
Same as pB165 but containing GST–GCN2(70–598)
|
this study |
| pB203 |
Same as pB165 but containing GST–GCN2(1–272)
|
this study |
| pB204 |
Same as pB165 but containing GST–GCN2(70–272)
|
this study |
| pB205 |
Same as pB165 but containing GST–GCN2(230–572)
|
this study |
| pB206 |
Same as pB165 but containing GST–GCN2(1–504) with codons 1–504 from D.melanogaster GCN2
|
this study |
| pB167 |
GST–GCN2(1–598), myc tagged in pB201 |
this study |
| pB201 |
LEU2 version of pEGKT |
this study and Mitchell et al. (1993)
|
| p897 |
tRNAHis in YEp13 |
Vazquez de Aldana et al. (1994) |
