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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: J Immunol. 2010 Oct 20;185(10):6277–6285. doi: 10.4049/jimmunol.1002330

Figure 4. Cytokine secretion after ischemia reperfusion or injection of LPS.

Figure 4

Panel A shows WT or Nlrp3−/− mice (n=6/gp) subjected to 25 min ischemia and indicated times of reperfusion. At the indicated time points plasma (for IL-18) or kidney homogenates (for IL-1 β) was assayed for the cytokines (top panels, IRI). Bottom panels show plasma cytokine secretion over time after LPS injection in WT (λ) or Nlrp3−/− (σ) mice (n=6/gp). The data are representative of 3 experiments with the same results. Error bars represent standard deviations. Panel B shows IL-1β and caspase 1 in WT vs. Nlrp3−/− kidneys from sham operated (sham) or IRI mice. The top panel shows mature IL-1β (mature IL-1β identified by the p17 fragment). The middle panel shows caspase 1 (active subunit p20) and the lower panel shows hsp-90 for a loading control. This represents one of 3 experiments with identical results.