Fig. 8.

Percentage change in body weight of CBA mice infected with mock inoculum, RacL11 EHV-1, and ΔIR3 EHV-1, and EHV-1 titers of mouse lungs. Mice were intranasally inoculated with sterile medium as control or with 2×10⁶ PFU/mouse of either wt RacL11 EHV-1 or ΔIR3 EHV-1, and total virus was isolated from mouse lungs and titered as described in Materials and Methods. Body weight was measured daily, and the two-tailed Student’s t test was used for data analysis to compare body weight between groups. Error bars indicate standard deviation. (A) Percentage change in body weight of CBA mice infected with ΔIR3 EHV-1 (n=9), IR3R (n=9) or RacL11 EHV-1 (n=9). (B) Percent survival of the mice infected with RacL11 EHV-1 (n=9), IR3R EHV-1 (n=9), ΔIR3 EHV-1 (n=9), or mock (n=9). (C) Viral titers of lungs of live mice infected with RacL11 EHV-1 (n=3, white bars), IR3R EHV-1 (n=3, black bars), or ΔIR3 EHV-1 (n=3, gray bars) at 2, 3, and 4 dpi. (D) Viral titers of lungs from dead mice infected with RacL11 EHV-1 (white bars), IR3R EHV-1 (black bars) or ΔIR3 EHV-1 (gray bars) at 2, 3, 4, 5, and 6 dpi. Each bar indicates the average viral titer of mice succumbed at the same day. The sample number(s) of each bar from at 2 dpi to 6 dpi are n=1 (bar 1), n=4 (bar 2), n=2 (bar 3), n=2 (bar 4), n=3 (bar 5), n=2 (bar 6), n=3 (bar 7), n=1 (bar 8), n=2 (bar 9), n=3 (bar 10), and n=1 (bar 11), respectively.