Table I. Splicing efficiency of various reporters in msl5 mutant and wild-type strains.
Strain | msl5-2 | msl5-3 | msl5-9 | MSL5-WT | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
reporter | WT | 5′ | BP | 5′ + BP | – | WT | 5′ | BP | 5′ + BP | – | WT | 5′ | BP | 5′ + BP | – | WT | 5′ | BP | 5′+ BP | – |
m/p | 11 | 0.4 | 3.2 | 0.3 | – | 24 | 0.4 | 4.6 | 0.3 | – | 23 | 1.1 | 4.1 | 0.02 | – | 34 | 5.8 | 15 | 1.6 | – |
Reporters contained the RP51A intron in the wild-type form (WT), with a mutation in the 5′ splice site (5′), in the branchpoint region (BP) or in both (5′ + BP). As a control for background from endogenous RNA, cells containing an empty vector (–) were analyzed. The signals corresponding to pre-mRNA and mRNA after primer extension were quantified and the ratio of mRNA/pre-mRNA (m/p) was calculated (Pikielny and Rosbash, 1985).