Table I. Apparent affinities of eIF-5A derivatives for Exp4–RanGTP complex.

eIF-5A species	Apparent KD for dissociation from eIF-5A–Exp4–RanGTP complex	Relative affinity for Exp4 (native eIF-5A = 100)
Purified from HeLa cells, fully hypusinated	2 nM	100
Recombinant, non-modified	75 nM	2.5
Recombinant, deoxyhypusinated	25 nM	8
Recombinant domain I (residues 1–83), deoxyhypusinated	∼1–2 µM	0.1
Recombinant domain II	>10 µM	<0.01