Fig. 7.
Illustration of sequence specific resonance assignment of a penta-peptide segment in Ubiquitin. The procedure described in Fig. 4 is repeated for three contiguous tri-peptide segments: Y59–N60–I61, N60–I61–Q62 and I61–Q62–K63. First, the 15N and 1HN resonances of N60 and I61 are identified with the procedure described in Fig. 4 using the (R, N) selectively unlabeled sample. Subsequently, the procedure is repeated to obtain the 15N and 1HN resonances of Q62 and K63 using the (K, L) and (Q, I) selectively unlabeled samples, respectively