Fig. 8.

a 2D [¹⁵N–¹H] HSQC and (b) a selected region of 2D {¹²CO_i–¹⁵N_i+1}-filtered HSQC acquired for lysine selectively unlabeled sample of an 11 kDa intrinsically disordered protein Tim23. In (a) overlay of 2D [¹⁵N–¹H] HSQC of the uniformly ¹⁵N labeled Tim23 (blue) with that of lysine selectively unlabeled (red) is shown. Assignments are indicated with the single letter code followed by the residue number. Note that the residue i + 1 of K25 is a Pro and hence not observed in the 2D {¹²CO_i–¹⁵N_i+1}-filtered HSQC