Table 1.

Bacterial strains and plasmids used in this study.

Shigella strains	Strain/plasmid	Reference
M90T	Wild-type M90T, serotype 5a	Sansonetti et al. (1982)
SF620	ipaB mutant	Menard et al. (1993)
mxiG	mxiG mutant	Allaoui et al. (1995)
mxiC	mxiC KO mutant	This study
mxiC/mxiC+	mxiC mutant/ pIMA208	This study
SH116	mxiH mutant	Blocker et al. (2001)
mxiHK69A	SH116/pRKmxiHK69A	Kenjale et al. (2005)
mxiHD73A	SH116/pRKmxiHD73A	Kenjale et al. (2005)
mxiC mxiH	Double mutant mxiC mxiH	This study
mxiC mxiHK69A	mxiC mxiH/pRKmxiHK69A	This study
ipaBΔ3	SF620/pDR2	Roehrich et al. (2010)
mxiC ipaB	Double mutant mxiC ipaB	This study
mxiC ipaBΔ3	mxiC ipaB/pDR2	This study
ipaB mxiH	Double mutant ipaB mxiH	This study
ipaB mxiH/ipaB+mxiH+	ipaB mxiH/pIMA216	This study
ipaB mxiH /ipaB+mxiHK69A	ipaB mxiH/pIMA218	This study
ipaB mxiH /mxiH+	ipaB mxiH/pRKmxiH	This study
ipaB mxiH /mxiHK69A	ipaB mxiH/pRKmxiHK69A	This study
M90T/ pRK2mxiH	Wild-type /pRK2mxiH	Veenendaal et al. (2007)
mxiC/pRK2mxiH	mxiC/pRK2mxiH	This study

Plasmids	Description	Reference
pDR1	pUC19-ipaB	Roehrich et al. (2010)
pDR2	pUC19-ipaB C-terminal three-amino-acid truncation	Roehrich et al. (2010)
pIMA208	mxiC gene cloned into pUC19	This study
pIMA216	ipaB and mxiH genes cloned into pUC19	This study
pIMA218	ipaB and mxiHK69A genes cloned into pUC19	This study
pKD46	Plasmid expressing lambda red recombinase	Datsenko and Wanner (2000)
pRKmxiH	mxiH cloned into a pUC18 derivative	Kenjale et al. (2005)
pRKmxiHK69A	mxiHK69A cloned into a pUC18 derivative	Kenjale et al. (2005)
pRKmxiHD73A	mxiHD73A cloned into a pUC18 derivative	Kenjale et al. (2005)
pRK2mxiH	Plasmid overexpressing mxiH	Kenjale et al. (2005)