Table 2.
Primers used in this study.
| Primer | Sequencea |
|---|---|
| mxiC_SalI | GCACGCGTCGACAACTATAAAGTAGGTGA TGTATGCTTG |
| mxiC_BamHI | CGCGGATCCCTGGATCACTTTTATCTCCTGTTATC |
| mxiH_RBS_PstI | ACTACTGCAGCTAACAGGAGGAATTACATATGAGTGTTACAGTACCGAATGATG |
| mxiH_XbaI | ACTATCTAGATTATCTGAAGTTTTGAATAATTG |
| mxiC_KO_tetF | CATTGGTTTCATACTTAAATTACTAACTATAAAGTAGGTGATGTATGCTTGATGTTAATTAAGACCCACTTTCATAATTAAGACCCACTTTCA |
| mxiC_KO_tetR | TGCTTAAGAAAAGACTGGATCACTTTTATCTCCTGTTATCTAGAAAGCTCCATATGAATATCCTCCTTACTAAGCACTTGTCTCCTG |
a
Restriction sites are underlined.