Table I. AMP-PNP affinity, stability and relative ATPase activity of ts mutants.

Construct	Kd AMP-PNP-Mg2+	Tm	ATPase at 37°C
	(µM)	(°C)	(% of wild type)
Wild type	33	65.2	100
T22I	110	61.4	456
A41V	154	65.1	1.3
T101I	37	63.6	9.4
A587T	37	63.6	94.3